The fplc instrument in the immune monitoring lab is used mainly for pmhc tetramer production. Ge amersham pharmacia akta fplc chromatography system the akta fplc is a preparative liquid chromatography system for fast and easy purification of proteins, the compact and flexible design with an expandable rack allows the use of longer chromatographic columns and automation kits, features up to 20mlmin flow rate and 5mpa maximum pressure, upc900 measures uvabsorption, conductivity. The ge akta fplc system passed all tests and was found to be fully functional. During sizedependent peptide separation, the column is. Our systems can be customized to include almost any configuration of autosamplers, detectors and pumps. In the fast protein liquid chromatography fplc method, medium pressure chromatography is used to separate peptides mainly according to size and charge. Fplc fast protein liquid chromatography shared resources. We are constantly receiving used fplc equipment of various makes, models, and purposes. Ge pharmacia akta purifier 100 gmi trusted laboratory. Lot of 2 pharmacia pump p500 chromatography fplc hplc. Ge life sciences gel filtration columns gel filtration.
In subsequent years, a continuous stream of improvements and innovations in gel filtration chromatography were introduced by pharmacia and, as it was later called, amersham biotech and now ge life sciences, a division of ge healthcare. Fplc stands for fast protein liquid chromatography a pharmacia product line. If depot service is the best option for you we will assist you with logistics to help you keep freight and rigging charges as low as possible. Fplc software puritychrom the puritychrom is a powerful software to control your fplc system. Actual item pictured enjoy free shipping for continental usa only.
Pumps have movement, but they seem sluggish when moving, although we do not know how to correctly use or test these. In subsequent years, a continuous stream of improvements and innovations in gel filtration chromatography were introduced by pharmacia and, as it was later called, amersham. Fplc computer control software chromatography data storage introduction the fast protein liquid chromatography or fplc is a semiautomatic microprocessor controlled machine used primarily for the separation of macromolecules 14. Comes with new lamp pump has also been newly rebuilt includes. Fplc follows usually ion exchange and gel filtration chromatography. Ge healthcare akta purifier 10 fplc system youtube. The fplc instrument is used to purify and analyze proteins. I also have several other pieces of hplc fplc equipment listed. We offer an assortment of refurbished and used liquid chromatography solutions ranging from hplc, lcms to fplc. Fast protein liquid chromatography an overview sciencedirect. Linked unit to unicorn operating system passed tested flowrate accuracy of p900 pump passed.
Fplc system general description the ge pharmacia fast protein liquid chromatography fplc system is used for methods development and the purification of large quantities of various bimolecular i. It does power on, no further testing has been done. Pharmacia lkbuvm ii chromatography wavelength detector unit. Fully tested pharmacia fplc for sale antique furniture. Aktafplc ge healthcare, pharmacia, contichrom chromacon, biosmb pall life sciences. The akta fplc is a preparative liquid chromatography system for fast and easy purification of proteins, the compact and flexible design with an expandable rack allows the use of longer chromatographic columns and automation kits, features up to 20mlmin flow rate and 5mpa maximum pressure, upc900 measures uvabsorption, conductivity.
Amersham biosciences akta fplc system marshall scientific. Food and drug administration and state and local regulatory agencies. Each order is for one new, open box, pharmacia xk 50100 tube. Instrument ships complete and ready to use, and ready for optional professional installation. Tests all cables and power cords full 90 day parts and labor warranty. Comes with the cu950 interface, and the akta stack has the following. Ge pharmacia biotech phc900 akta hplc 18110776 monitor. P500 pump, gradifrac fraction collector with 96tube collection rack, lkb optical unit uv1 with control unit, rec 102 chart recorder, 5 mpa mixer module, mounting stand, and various cablesaccessories. Ge healthcare pharmacia biotech akta fplc p960 sample pump 2. Our hplc systems such as the agilent 1100 and 1200 series, akta fplcs, and. Refurbished amersham pharmacia biotech akta purifier 10.
The sale of this item may be subject to regulation by the u. Used pharmacia diagnostics equipment buy and sell equipnet. Industrial and laboratory equipment ebay shop add to favorites for any additional details, photos or expedited delivery please call or click ask a question at the bottomleft of listing descriptionhere is a ge healthcare pharmacia biotech akta fplc p960 sample pump 2. Agilenthp 1100 series hplc system w software, misc.
System requirements are an ibm pc or fully compatible microcomputer, mouse, cga or ega card and gem desktop software. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid the mobile phase and a porous solid the stationary phase. Please allow 2 week lead time for this system you should know. Akta explorer 100 air with computer loaded with unicorn software v5. The ge akta explorer air 100 chromatography systems, officially from amersham pharmacia biotech offer fast and reliable solutions for developing and optimizing any biomolecular purification strategy. Lot of 2 pharmacia pump p500 chromatography fplc hplc parts only click on images for larger view here is a lot of 2 pharmacia pump p500 chromatography fplc hplc. Fast protein liquid chromatography fplc scientific. Select a column even if you want to perform a test run without. Fast protein liquid chromatography fplc was performed as a second. Amersham biosciences akta prime fplc system laboratory. Fplc lab scale akta explorer 10s 2002 fraction collector frac950, many accessories,computer,software unicorn v.
Depending on the type of the column used, proteins are separated on the properties of size, charge, hydrophobicity, and so on. This ge amersham akta explorer 100 air fplc system is in good working order. Akta avant is a preparative protein purification chromatography system. It should be noted that researchers often use the terms fast protein liquid chromatography, fplc, and mediumpressure chromatography interchangeably. The akta purifier 100 is perfect for application spanning a wide range of wavelengths the akta purifier 100 allows for rate flows of up to 100 ml per minute fast and easy start to purification work preprogrammed application protocols and method templates speed and reliability for every run fast optimisation and peak identificationaccurate result comparisons and peakfractionation column. Onsite repair service available nationwide for pharmacia amersham 4050 lkb iii spectrophotometers. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials. Amino acids play central roles both as building blocks of proteins and as intermediates in metabolism. Pharmacia biotech akta fplc explorer 100 system with fraction. Refurbished amersham pharmacia biotech akta fplc system.
The amersham biosciences akta fplc system is a biocompatible, high performance liquid chromatography system for the fast and easy purification of proteins. Ge amersham akta explorer 100 air fplc unit2 quality used. Ge amersham pharmacia akta fplc chromatography system. This ge amersham akta explorer fplc is in good working order. Ge akta explorer certified fplc system gmi trusted lab. Ge akta pilot liquid chromatography system gmi cerfied. Fplc is defined as fast protein liquid chromatography a pharmacia product line very frequently. Fast protein liquid chromatography fplc system knauer. Get familiar with puritychrom in shortest time and with no effort due to the intuitive and clearly structured user interface. Fplc was introduced in 1982 by pharmacia as fast performance liquid chromatography.
Ge healthcares formerly amersham biosciences akta fplc. An image to pharmacia moons hr ml 10 mpa lab scale glass column for fplc system model. Click here to view the technical specs for the akta fplc system. Ge akta explorer amersham pharmacia certified fplc system gmi.
Ge pharmacia biotech phc 900 akta hplc 18110776 monitor with probe holder here is a ge pharmacia biotech phc 900 akta hplc 18110776 monitor with probe holderpredecessor to. Expansive selection of fully tested pharmacia fplc at an array of sticker prices. Pharmacia biotech akta fplc explorer 100 system with. The ge akta explorer chromatography systems, formally from amersham pharmacia biotech offer fast and reliable solutions for developing and optimizing any biomolecular purification scheme. Govdeals is the place to bid on government surplus and unclaimed property including heavy equipment, cars, trucks, buses, airplanes, and so much more. Some of our leading manufacturers include agilent, hp, waters, ge, amersham, dionex and shimazdzu. Gel filtration chromatograhy of proteins was first practiced in 1959 by jerker porath and per flodin at uppsala university and was subsequently commercialized by pharmacia fine chemicals under the trade name sephadex. The ge pharmacia fast protein liquid chromatography fplc system is used for methods development and the purification of large quantities of various bimolecular i. For optimal results, use prepacked columns, such as the hitrap, hiprep, and hiload columns that simply snap onto the side of the unit. Amersham ge pv908 pv908 akta valve amersham biosciences amercham pharmacia fplc fast protein liquid chromatography system features. Amersham pharmacia biotech akta fplc fast protein liquid chromatography 60 day warranty completely refurbished and functionally verified. Upon request, we can also run other protein samples if it is. Preliminary testing is conducted to identify potential product defects, upon completion, we replace all faulty andor underperforming parts with original manufacturer parts that perform up to specification. The ge akta fplc system passed all tests and was found to be fully.
Used amersham pharmacia akta flash liquid chromatographhplc. Related, high performance liquid chromatography affinity chromatography. Pharmacia xk 50100 liquid chromatography column xk50100. Ge healthcares akta fplc system biocompare product. Though i did not test these for lack of equipment, as a new item its assumed that they are working. Image is taken from the ngc systems chromlab software. Fast protein liquid chromatography fplc is a form of mediumpressure chromatography that uses a pump to control the speed at which the mobile phase passes through the stationary phase. Pharmacia biotech 80630116 hoefen sq3 sequencer unused. Tested switching valves and verified flow rates for both pumps. Used amersham pharmacia akta explorer 100 ge akta explorer. Refurbished ge healthcare life sciences akta fplc system. The ge amersham akta explorer 100 air fplc system passed all tests and was found to be fully functional. The akta fplc chromatographic system is a redesign of the classic pharmacia fplc instrument, the gold standard in protein purification for over 20 years.
Protein is the collection of aminoacid moieties and essential part of human nutrition. Start off by verify your companys address, fill in appropriate boxes, then click submit. Post a free request for proposal for a amersham pharmacia akta explorer 100 ge akta explorer 100 air fplc system frac 950, cu950 and get quotes from our gold service dealers. Fast protein liquid chromatography fplc, is a form of liquid chromatography that is often used. Both pumps power on and both pumps move and valve works. The akta fplc is a preparative liquid chromatography system for fast and easy purification of proteins, the compact and flexible design. Hplc and fplc fplc fast protein liquid chromatography.
Using the integrated doe functionality of the unicorn software, the number of. There is some cosmetic flaking of the paint on the top of the unit. Equipnet is the worlds leading provider of used fplc equipment and various other equipment. This instrument has been tested by our inhouse service technician and is ready for use. Ge amersham akta explorer 100 fplc system asa global medical. Showcasing fully tested pharmacia fplc on sale right now on the internet. The 20 amino acids that are found within proteins convey a vast array of chemical versatility. Pharmacia fplc system this column chromatography system automates the separation and purification of semipreparative amounts of the compound of interest, typically a protein. Ge amersham akta fplc flash purification liquid chromatography pred to avant predecessor to. Unit has been powered up to test, no further testing has been done. Please scroll down to see the fullsize images of the actual unit available. However, an elementary fplc system is basically composed by.
Pharmacia biotech lkb fplc hplc frac200 fraction collector with psv30 valve item comes from a working environment. The system uses aqueous buffers and fairly low pressures, and is housed in a cold box so that chromatographic runs can be done at 4c, thus helping to preserve biological activity. Unicorn, drop design, fplcdirector, oligopilot, bioprocess, ettan, akta, aktaxpress. Find great deals on a huge selection of ge healthcare akta in auctions, and classified for sale ads on labx. Amersham pharmacia akta explorer 100 fplc system at. The only difference between fplc and hplc is the amount of pressure the pumps apply to the column.
The system simplifies the transition from laboratory to full scale production. Fast protein liquid chromatography fplc, is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. One box was opened, the other was not, but i opened it to check for condition, and both are identical. Ge amersham akta explorer 100 fplc chromatography systems are made for fast development and optimization of almost any biomolecular purification, utilizing one working platform for all samples and techniques.
Amersham biosciences akta prime fplc system is available from laboratory controls llc. Ge pharmacia biotech phc 900 akta hplc 18110776 monitor with probe holder here is a ge pharmacia biotech phc 900 akta hplc 18110776 monitor. Ge healthcare life sciences columns and media hplc. Apr 18, 2016 fplcfast protein liquid chromatography 1. The new system is build around the akta design platform common for all of the ge healthcare chromatographic instruments, from lowpressure aktaprime to the highperformance aktapurifier. This system offers fast and reliable solutions for developing and optimizing any biomolecular purification scheme. The precise amino acid content, and the sequence of those amino acids, of a specific protein, is determined by. How is fast protein liquid chromatography a pharmacia product line abbreviated. I find the software provided with the instrument extremely easy to handle. Pc loaded with unicorn software and communication interface. Pharmacia biotech akta fplc explorer 10 system w datastation. P900 pump, uv900 uv detector, phc conductivity monitor, the a900 autosampler, and box900. Our exclusive contracts with our clients yield a wide range of used fplc equipment from a number of respected oems, including ge healthcare, pharmacia biotech, amersham pharmacia biotech inc, and many others. The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers.
Chromatographic techniques at flow rates and pressures compatible with modern separation media are used to produce highcaliber solutions. Included software was already configured, making setup easier than expected. Akta avant cytiva formerly ge healthcare life sciences. Ge healthcare pharmacia biotech akta fplc p960 sample. Includes 1 ge amersham pharmacia biotech akta fplc pump p920. Fplc columns have a maximum pressure of about of 34 mpa, whereas hplc columns can withstand or require much higher pressures. Ge amersham pharmacia akta fplc chromatography system in mountain view, ca, usa. Providing flexibility and reliability in every run, the akta provides a unique combination of flow configuration and methods support for all chromatographic techniques including gel filtration size exclusion chromatography, anion exchange. Ge akta explorer amersham pharmacia certified fplc system. Since then, many different mediumpressure chromatography systems have been developed. Call us today to learn more about the ge amersham pharmacia akta pilot, our service product offerings on the entire akta product line, a personalized pricing proposal, or how to purchase a brand new production biochromatography fplc system.
Software used as part of clinical study is assessed against the compliance level of the application for 21 cfr part 11 and the risk related to this application. Used amersham pharmacia biotech akta purifier fplc system in granite quarry, nc. Fplc fast protein liquid chromatography a pharmacia. Fplc fast protein liquid chromatography or fast performance liquid chromatography 2. Contents introduction principle instrumentation advantages drawbacks application conclusion 3. Computer control of fast protein liquid chromatography fplc. Ge healthcare akta purifier 10 fplc system available from the lab world group. The fraction collector is typically a rotating rack that can be filled with test. The following onboard tests were performed to the main components p900 pump uv900 detector. Ge akta prime plus fplc system marshall scientific. You can register on our website using the exact company name that is on your companys w9 or corporate return. The method magician and optimized system configurations enable rapid networking viewing, method scouting, procedure optimization, and also scaleup experiments.
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